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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
中乔新舟
- 品系:
细胞系
- 运输方式:
常温
- 年限:
液氮长期
- 生长状态:
贴壁生长
- 规格:
T25
|
产品名称 |
U-138MG人神经星形胶质母细胞瘤 |
|
货号 |
ZQ0927 |
|
产品介绍 |
U-138MG人神经星形胶质母细胞瘤是 J. Ponten 及其同事于 1966 年至 1969 年从恶性神经胶质瘤衍生的许多细胞系之一(另见ATCC HTB-14和ATCC HTB-15)。它在形态上与ATCC HTB-14不同,并且增殖速度较慢。到 1974 年 3 月观察到支原体污染并治愈。注:据报道,来自不同个体的两种胶质母细胞瘤细胞系 U-118 MG (HTB-15) 和 U-138 MG (HTB-16) 具有相同的 VNTR 和相似的 STR 模式。U-118 MG 和 U-138 MG 在细胞遗传学上非常相似,并且共享至少六个衍生标记染色体。 |
|
种属 |
人 |
|
性别/年龄 |
男/47岁 |
|
组织 |
脑 |
|
疾病 |
胶质母细胞瘤;Ⅳ级 |
|
细胞类型 |
肿瘤细胞 |
|
形态学 |
多边形的 |
|
生长方式 |
贴壁 |
|
倍增时间 |
47 hours (PubMed=9842975); ~70 hours (DSMZ=ACC-291) |
|
培养基和添加剂 |
DMEM (中乔新舟 货号:ZQ-100)+10%胎牛血清(中乔新舟 货号:ZQ500-A)+1%P/S(中乔新舟 货号:CSP006) |
|
推荐完全培养基货号 |
ZM0927 |
|
生物安全等级 |
BSL-1 |
|
培养条件 |
95%空气,5%二氧化碳;37℃ |
|
STR位点信息 |
Amelogenin: X,Y CSF1PO: 12
|
|
抗原表达/受体表达 |
*** |
|
基因表达 |
*** |
|
保藏机构 |
ATCC; HTB-16;BCRJ; 0239;DSMZ; ACC-291 |
|
供应限制 |
仅供科研使用 |
上海中乔新舟生物科技有限公司成立于2011年,历经十多年发展,主要专注于细胞生物学产品的研究和开发,专注于为药企、各类科研机构及CRO企业提供符合标准规范的细胞培养服务、细胞培养基、细胞检测试剂盒、细胞培养试剂,胎牛血清和细胞生物学技术服务等。
公司一直致力于为高等院校、研究机构、医院、CRO及CDMO企业提供细胞培养完整解决方案,这些产品旨在满足细胞培养的多样需求,确保实验和研究的有效进行。引用中乔新舟(ZQXZBIO)产品和服务的文献超数千篇。

产品服务
细胞资源:原代细胞、细胞株、干细胞、示踪细胞、耐药株细胞、永生化细胞等基因工程细胞。
试剂产品:胎牛血清、完全培养基(适用于原代细胞及细胞株)、无血清培养基、基础培养基、细胞转染试剂、重组因子、胰酶和双抗等等细胞培养所有实验相关产品。
技术服务:稳转株构建、原代细胞分离、特殊培养基定制服务、细胞检测等。

目前产品已经畅销国内30多个省市,与客户建立长期的合作伙伴关系,共同实现成功。全体员工将不懈努力,继续为科研人员提供优良的产品和服务,致力成为全球细胞培养领域的参与者。

企业愿景
致力于成为国内细胞培养基产业的佼佼者,生物医药领域上游原材料的优良提供商。
企业使命
成长为专业细胞系及原代细胞培养供应商、专业细胞培养基及培养试剂生产商。
企业荣誉


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Long term culture of normal and neoplastic human glia.
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Macintyre E.H., Ponten J., Vatter A.E.
The ultrastructure of human and murine astrocytes and of human fibroblasts in culture.
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The deficient density-dependent growth control of human malignant glioma cells and virus-transformed glia-like cells in culture.
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Tumor-specific membrane antigens in established cell lines from gliomas.
Cancer 34:274-279(1974)
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Ponten J.
Neoplastic human glia cells in culture.
(In) Human tumor cells in vitro; Fogh J. (eds.); pp.175-206; Springer; New York (1975)
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Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
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Human brain tumour cell strains with deficient host-cell reactivation of N-methyl-N'-nitro-N-nitrosoguanidine-damaged adenovirus 5.
Nature 279:797-799(1979)
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Human tumor cell strains defective in the repair of alkylation damage.
Carcinogenesis 1:21-32(1980)
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Heterogeneity of genotypic and phenotypic characteristics of fifteen permanent cell lines derived from human gliomas.
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Polymorphic enzyme analysis of cultured human tumor cell lines.
J. Natl. Cancer Inst. 70:469-476(1983)
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Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.
Proc. Natl. Acad. Sci. U.S.A. 81:568-572(1984)
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Human tumor lines for cancer research.
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Cancer Res. 48:3910-3918(1988)
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Data Brief 34:106643.1-106643.13(2021)
1.材料和方法1.1 DMEM/F12条件培养液(亚硒酸钠40μg·L-1,腐胺16.2 mg·L-1,转铁蛋白100 mg·L-1,胰岛素234U·L-1,甲状腺素0.4μg·L-1,黄体酮0.62 mg·L-1,谷氨酰胺3g·L-1)。DMEM/F12培养基、小牛血清购自Hyclone公司,兔抗鼠GC抗体、亚硒酸钠、腐胺等购自Sigma公司。1.2 视神经胶质细胞的来源、培养 取新生2d的Wistar大鼠10只(第三军医大学大坪医院野战外科研究所动物中心提供),无菌条件下取出双
腺瘤细胞 SMMC-7721 人肝癌细胞 SW 13 人肾上腺皮质瘤 SW480 人结直肠癌 T84 人结肠癌细胞 TALL104 IL-2依赖株(B类) TF1 人红系白血病细胞(B类) THP1 人单核细胞型淋巴瘤 U-2 OS 人骨肉瘤细胞 U251 人神经胶质细胞瘤 U-937 人淋巴瘤细胞 UACC812 人乳腺导管瘤 UT-7 人类原巨核细胞白血病细胞(B类) ZR-75-1 人乳腺导管癌
【精华】vol 687 Chapter 4 巢式PCR侧翼序列测定实验
in the presence of 2 mM manganesechloride and 0.1 mg/ml bovine serum albumin at 42°C for1 h in a total reaction volume of 20 ml (see Note 9). 2. Reverse transcription products are treated with 2 U RNaseH at37°C for 20 min prior to heat inactivation at 85°C
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