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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科技有限公司
- 检测范围:
6.25pg/mL
- 检测方法:
Sandwich
- 应用:
Cell Biology
- 适应物种:
Homo sapiens (Human)
- 样本:
serum, plasma, tissue homogenates, cell lysates
- 规格:
96T/48T
| 规格: | 96T | 产品价格: | ¥3600.0 |
|---|---|---|---|
| 规格: | 48T | 产品价格: | ¥2500.0 |
| Product Name |
Human Tripartite motif-containing protein 72(TRIM72) ELISA Kit |
| Chinese Name |
人三结构域包含蛋白72(TRIM72)ELISA Kit |
| Code |
CSB-EL024511HU |
| Alternative Names |
LOC493829 ELISA Kit; Mg53 ELISA Kit; Mitsugumin-53 ELISA Kit; TRI72_HUMAN ELISA Kit; trim72 ELISA Kit; Tripartite motif containing 72 ELISA Kit; Tripartite motif containing protein 72 ELISA Kit; Tripartite motif-containing protein 72 ELISA Kit |
| Target Name |
tripartite motif-containing 72 |
| Abbreviation |
TRIM72 |
| Uniprot No. |
Q6ZMU5 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
25pg/mL-1600pg/mL |
| Sensitivity |
6.25pg/mL |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450nm |
| Research Area |
Cell Biology |
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文献和实验Mapping Protein‐Protein Interactions with Phage‐Displayed Combinatorial Peptide Libraries
by purification of selected phage by ELISA. Alternatively, there is a bead?based affinity selection method. These methods allow one to readily isolate peptide ligands that bind to a protein target of interest and use the consensus sequence to search proteomic
Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
the throughput of assays capable of detecting transient extracellular protein interactions. These methodological improvements now permit screening for novel extracellular receptor?ligand interactions on a genome?wide scale. Curr. Protoc. Protein Sci. 72:27.5.1?27
【专题讨论】杆状病毒——昆虫细胞蛋白表达系统,影响蛋白表达的因素?
方法扩增P3贮液,用于高效表达。 三、 病毒贮液初步鉴定 1. SDS-PAGE蛋白电泳:取P2或P3病毒贮液浓缩后上样(或直接上样)进行SDS-PAGE蛋白电泳,初步根据分子量对表达蛋白进行鉴定。 CAT-his融合蛋白分子量约为28KDa,nsp6-his融合蛋白分子量约为34KDa。 2. western blot:以小鼠抗his单克隆抗体鉴定在相应条带出是否有his标记。 3. 以CAT-ELISA检测试剂盒检测Bacmid/CAT对照的蛋白表达情况。方法详见CAT
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