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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科技有限公司
- 检测范围:
0.195ng/mL
- 检测方法:
Sandwich
- 应用:
Immunology
- 适应物种:
Homo sapiens (Human)
- 样本:
serum, plasma, tissue homogenates
- 规格:
96T/48T
| 规格: | 96T | 产品价格: | ¥3600.0 |
|---|---|---|---|
| 规格: | 48T | 产品价格: | ¥2500.0 |
| Product Name |
Human Complement Fragment 4d,C4d ELISA Kit |
| Chinese Name |
人补体片段4d(C4d)ELISA Kit |
| Code |
CSB-E14192h |
| Target Name |
Complement Fragment 4d,C4d |
| Abbreviation |
C4d |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.78ng/mL-50ng/mL |
| Sensitivity |
0.195ng/mL |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450nm |
| Research Area |
Immunology |
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文献和实验The definition of HLA-specific antibodies in solid organ transplant patients is a necessary tool for recipient selection prior to transplantation and monitoring for rejection post transplant. Solid phase assays can detect both complement
Human Complement Components C4A and C4B Genetic Diversities: Complex Genotypes and Phenotypes
van den Elsen, J.M.H., Martin, A., Wong, V., Clemenza, L., Rose, D.R., and Isenman, D.E. 2002. X‐ray crystal structure of the C4d fragment of human complement component C4. J. Mol. Biol. 322:1103‐1115
7)作为包被抗体,以识别和结合待检标本中的IL-8,另一株作为酶标抗体,与结合于包被抗体的IL-8的另一个表位结合并催化底物显色。二、试剂器材1. 试剂盒提供包被抗体、酶标抗体、标准品,底物(ABTS)、96孔ELISA板。2. 包被缓冲液、PBS、底物缓冲液配制同常规ELISA法。三、操作步骤1. 包被:pH9.5 碳酸盐缓液稀释4D7单克隆抗体粗提γ球蛋白至1μg/ml,加入96孔板,100μl/孔,放4℃,36小时。2. 封闭:0.1%吐温PBS(Buffer A)冲洗96孔板三次,加3%BSA
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