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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科技有限公司
- 检测范围:
3.9pg/mL
- 检测方法:
Sandwich
- 应用:
Epigenetics and Nuclear Signaling
- 适应物种:
Homo sapiens (Human)
- 样本:
serum, plasma, tissue homogenates, cell lysates
- 规格:
96T/48T
| 规格: | 96T | 产品价格: | ¥3600.0 |
|---|---|---|---|
| 规格: | 48T | 产品价格: | ¥2500.0 |
| Product Name |
Human ADP-ribosylation factor-like protein 8B(ARL8B) ELISA Kit |
| Chinese Name |
人ADP核糖基化因子样蛋白8B(ARL8B)ELISA Kit |
| Code |
CSB-EL002100HU |
| Alternative Names |
ADP ribosylation factor like 10C ELISA Kit; ADP ribosylation factor like 8B ELISA Kit; ADP-ribosylation factor-like protein 10C ELISA Kit; ADP-ribosylation factor-like protein 8B ELISA Kit; ARL10C ELISA Kit; ARL8B ELISA Kit; ARL8B_HUMAN ELISA Kit; FLJ10702 ELISA Kit; Gie1 ELISA Kit; Novel small G protein indispensable for equal chromosome segregation 1 ELISA Kit |
| Target Name |
ADP-ribosylation factor-like 8B |
| Abbreviation |
ARL8B |
| Uniprot No. |
Q9NVJ2 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
15.6pg/mL-1000pg/mL |
| Sensitivity |
3.9pg/mL |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450nm |
| Research Area |
Epigenetics and Nuclear Signaling |
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文献和实验Assay for Protein Modification by Poly-ADP-Ribose In Vitro
The enzymatic function of poly(adenosine diphosphate (ADP)-ribose) polymerase (PARP) is central to many of its function as a component of DNA repair machinery, modulator of gene transcription, and cell differentiation. While the auto
Generation of Poly(ADP-ribosyl)ation Deficient Mutants of the Transcription Factor, CTCF
to produce such mutants, demonstrated by the example of CTCF, a transcription factor. To achieve this in our study, the protein domain modified by PARylation was mapped and the amino acids, which can be potentially PARylated, selected. Mutations
特异性的引物PCR扩增。基因经过30-35个循环的一轮PCR扩增。50μl PCR扩增产物中的15μl在1.2%E-Gel®琼脂糖凝胶电泳检测,在紫外光下观察。 泳道1:HPRT(次黄嘌呤磷酸核糖转移酶),1.3kb,30拷贝/细胞 泳道2:SMAP(胸腺激素受体共激活蛋白),3.1kb 泳道3:Cleavage and polyadenylation specificity factor,4.5kb 泳道4:TFRC(转铁蛋白受体),5.0kb 泳道5:IGFⅡR(类胰岛素生长因子Ⅱ受体
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