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发货周期:现货
博士德生物
cell culture supernatants, serum and plasma (heparin, citrate).
Human
96T
货号 | FEK1123 |
---|---|
产品名称 | Human CCL14/HCC-1 Quick ELISA Kit |
产品描述 | The Quick Picokine ELISA kits, assay takes less than 1.5 hours. Detect human CCL14 with <1 pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up. |
免疫原 | Expression system for standard: E.coli; Immunogen sequence: T20-N93 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10 pg/ml |
检测范围 | 15.6 pg/ml - 1,000 pg/ml |
基因名称 | CCL14 |
基因全名 | chemokine (C-C motif) ligand 14 |
Uniprot ID | CCL14: Q16627 |
背景资料 | Chemokine(C-C motif) ligand 14(CCL14) is a small cytokine belonging to the CC chemokine family. It is also commonly known as HCC-1. It is produced as a protein precursor that is processed to generate a mature active protein containing 74 amino acids that and is 46% identical in amino acid composition to CCL3 and CCL4. This chemokine is expressed in various tissues including spleen, bone marrow, liver, muscle, and gut. CCL14 activates monocytes, but does not induce their chemotaxis. In addition, HCC-1 enhanced the proliferation of CD34+ myeloid progenitor cells. It was as effective as MIP-1 alpha, but about 100-fold less potent. Human CCL14 is located on chromosome 17 within a cluster of other chemokines belonging to the CC family. |
显色时间 | 15分钟 |
SKU | 名称 | 数量 |
FEK1123-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 10 ng/tube |
EK1123-ST | Human CCL17/TARC Standard | 10ng/tube×2 |
FEK1123-C | Human CCL17 Antibody Cocktail | 6ml |
AR1106-1 | Sample Diluent | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
PLA-SEA | Adhesive cover | 2 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 42 | 232 | 450 | 45 | 232 | 451 |
Standard deviation | 2.14 | 12.99 | 25.20 | 2.97 | 17.16 | 34.72 |
CV(%) | 5.1% | 5.6% | 5.6% | 6.6% | 7.4% | 7.7% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 |
O.D. | 0.023 | 0.092 | 0.163 | 0.208 | 0.563 | 1.085 | 1.851 | 2.381 |
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