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发货周期:现货
博士德生物
cell culture supernatants, serum and plasma (heparin, citrate).
Human
96T
货号 | FEK0817 |
---|---|
产品名称 | Human Kallikrein 5/KLK5 Quick ELISA Kit |
产品描述 | The Quick Picokine ELISA kits, assay takes less than 1.5 hours. Detect human KLK5 with <10 pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernatants, serum, plasma (heparin, EDTA), human milk and saliva. This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up. |
免疫原 | Expression system for standard: NS0; Immunogen sequence: V23-S293 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10 pg/ml |
检测范围 | 78 pg/ml - 5,000 pg/ml |
基因名称 | KLK5 |
基因全名 | kallikrein-related peptidase 5 |
Uniprot ID | KLK5: Q9Y337 |
背景资料 | Kallikrein gene 5(KLK5, also known as KLK-L2), located on chromosome 19q13.4, is one of the newly identified members of the kallikrein gene family, which is a subgroup of the serine protease enzyme family. In normal human tissues, KLK5 is highly expressed in skin, mammary gland and testis. KLK5 has been suggested to regulate cell shedding(desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14. |
显色时间 | 15分钟 |
SKU | 名称 | 数量 |
FEK0817-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 10 ng/tube |
EK0817-ST | Human CCL17/TARC Standard | 10ng/tube×2 |
FEK0817-C | Human CCL17 Antibody Cocktail | 6ml |
AR1106-1 | Sample Diluent | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
PLA-SEA | Adhesive cover | 2 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 139 | 814 | 2166 | 151 | 752 | 2373 |
Standard deviation | 5.69 | 35.81 | 121.29 | 9.96 | 38.35 | 151.87 |
CV(%) | 4.1% | 4.4% | 5.6% | 6.6% | 5.1% | 6.4% |
Concentration(pg/ml) | 0 | 78 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 |
O.D. | 0.033 | 0.096 | 0.150 | 0.312 | 0.430 | 0.798 | 1.315 | 1.913 |
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