手机验证
发货周期:7-8周
博士德生物
cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
Human
96T
货号 | EK2159 |
---|---|
产品名称 | Human CD34 ELISA Kit |
产品描述 | For quantitative detection of human CD34 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
免疫原 | Expression system for standard: NS0; Immunogen sequence: S32-T290 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10 pg/ml |
检测范围 | 156 pg/ml - 10,000 pg/ml |
基因名称 | CD34 |
基因全名 | CD34 molecule |
Uniprot ID | CD34: P28906 |
指标别名 | CD34; Hematopoietic progenitor cell antigen CD34; CD antigen CD34 |
背景资料 | CD34 is a transmembrane phosphoglycoprotein protein encoded by the CD34 gene in humans, mice, rats and other species. The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK2159-CAP | 96-well plate precoated with anti- Human CD34 antibody | 1 |
EK2159-ST | Lyophilized recombinant Human CD34 standard | 10ng/tube×2 |
EK2159-DA | Biotinylated anti- Human CD34 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。Intra-Assay Precision | Inter-Assay Precision | ||||||
Sample 1 | 752 | 769 | 782 | 810 | 778 | 23.35 | 3.0% |
Sample 2 | 2754 | 2655 | 2896 | 2746 | 2763 | 176.82 | 6.4% |
Sample 3 | 6023 | 5940 | 6120 | 6454 | 6134 | 276.04 | 4.5% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
O.D. | 0.037 | 0.079 | 0.180 | 0.365 | 0.824 | 1.269 | 1.855 | 2.352 |
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