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发货周期:7-8周
博士德生物
cell culture supernatants, serum and plasma (heparin, EDTA).
human
96T
货号 | EK2217 |
---|---|
产品名称 | Human B7-H2/ICOSL ELISA Kit |
产品描述 | For quantitative detection of human ICOSLG in cell culture supernatants, serum and plasma (heparin, EDTA). |
免疫原 | Expression system for standard: NS0; Immunogen sequence: D19-S258 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <300 pg/ml |
检测范围 | 625 pg/ml - 40,000 pg/ml |
基因名称 | ICOSLG |
基因全名 | inducible T cell costimulator ligand |
Uniprot ID | ICOSLG: O75144 |
指标别名 | ICOSLG; B7H2; B7RP1; ICOSL; KIAA0653; ICOS ligand; B7 homolog 2; B7-H2; B7-like protein Gl50; B7-related protein 1; B7RP-1; CD antigen CD275 |
背景资料 | ICOS ligand (ICOSLG, ICOSL or GL50) is a protein that in humans is encoded by the ICOSLG gene located at chromosome 21. Enables identical protein binding activity. Predicted to be involved in T cell receptor signaling pathway and positive regulation of interleukin-4 production. Located in cytoplasmic ribonucleoprotein granule and plasma membrane. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK2217-CAP | 96-well plate precoated with anti- Human B7-H2/ICOSL ELISA Kit A antibody | 1 |
EK2217-ST | Lyophilized recombinant Human B7-H2/ICOSL ELISA Kit A standard | 40ng/tube×2 |
EK2217 -DA | Biotinylated anti- Human B7-H2/ICOSL ELISA Kit A antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 2015 | 4613 | 23467 | 1987 | 4563 | 21547 |
Standard deviation | 90.68 | 281.39 | 1608 | 97.36 | 333.1 | 1335.91 |
CV(%) | 4.5% | 6.1% | 6.8% | 4.9% | 7.3% | 6.2% |
Concentration(pg/ml) | 0 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 | 40000 |
O.D. | 0.019 | 0.115 | 0.193 | 0.410 | 0.737 | 1.176 | 1.355 | 2.053 |
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