The Multiplex Human Cytokine HS (9-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 9 biomarkers or analytes. Using less than 50 µl of sample per well, up to 80 samples can be assayed for all 9 markers in the panel within 2.25 hours. The Multiplex Human Cytokine HS (9-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 9 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.
Assay Ranges
Units
Calibrator Range
LLD Limit
IL-1α
pg/ml
4000 – 5.49
5.43
IL-1β
pg/ml
10000 – 13.72
10.14
IL-2
pg/ml
4000 – 5.49
3.16
IL-4
pg/ml
2000 – 2.74
2.14
IL-6
pg/ml
3000 – 4.11
3.47
IL-8
pg/ml
2000 – 2.74
2.70
IL-10
pg/ml
3000 – 4.11
4.10
IFNγ
pg/ml
8000 – 10.97
4.95
TNFα
pg/ml
4000 – 5.49
5.20
Kit Components
Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format please contact us for customization.
Reagents Include:
Calibrator: Lyophilized, recombinant antigens in a buffered protein base Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant Plate Seals (20): Adhesive strips