E.coli-derived human PLCB1 recombinant protein (Position: V888-E1059). Human PLCB1 shares 99% amino acid (aa) sequence identity with both mouse and rat PLCB1.
内容
500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
PLCB1 is also known as PLC-I, EIEE12 or PLC154. The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. This gene is activated by two G-protein alpha subunits, alpha-q and alpha-11. Two transcript variants encoding different isoforms have been found for this gene.
研究类别
1. Guo Y, et al. A loss in cellular protein partners promotes α-synuclein aggregation in cells resulting from oxidative stress. Biochemistry, 2013 Jun 4.2. Ngoh A, et al. Severe infantile epileptic encephalopathy due to mutations in PLCB1: expansion of the genotypic and phenotypic disease spectrum. Dev Med Child Neurol, 2014 Nov.3. Philip F, et al. Role of phospholipase C-β in RNA interference. Adv Biol Regul, 2013 Sep.
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
[list_product_images]Figure 1. Western blot analysis of PLCB1 using anti-PLCB1 antibody (PB0360). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate,Lane 2: Mouse Brain Tissue Lysate,Lane 3: Mouse Testis Tissue Lysate,Lane 4: Human Placenta Tissue Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLCB1 antigen affinity purified polyclonal antibody (Catalog # PB0360) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLCB1 at approximately 139KD. The expected band size for PLCB1 is at 139KD.[/list_product_images]