A synthetic peptide corresponding to a sequence at the C-terminus of human MPP1 (409-450aa TEALQQLQKDSEAIRSQYAHYFDLSLVNNGVDETLKKLQEAF), different from the related mouse sequence by one amino acid.
内容
500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
55 kDa erythrocyte membrane protein is a protein that in humans is encoded by the MPP1 gene. This gene encodes the prototype of the membrane-associated guanylate kinase (MAGUK) family proteins. MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intercellular junctions. The encoded protein is an extensively palmitoylated membrane phosphoprotein containing a PDZ domain, a Src homology 3 (SH3) motif, and a guanylate kinase domain. This gene product interacts with various cytoskeletal proteins and cell junctional proteins in different tissue and cell types, and may be involved in the regulation of cell shape, hair cell development, neural patterning of the retina, and apico-basal polarity and tumor suppression pathways in non-erythroid cells. Multiple transcript variants encoding different isoforms have been found for this gene.
研究类别
1. Bryant PJ, Woods DF (1992). "A major palmitoylated membrane protein of human erythrocytes shows homology to yeast guanylate kinase and to the product of a Drosophila tumor suppressor gene". Cell 68 (4): 621–2.2. Ruff P, Speicher DW, Husain-Chishti A (Sep 1991)."Molecular identification of a major palmitoylated erythrocyte membrane protein containing the src homology 3 motif". Proc Natl Acad Sci U S A 88 (15): 6595–9.
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
[list_product_images]Western blot analysis of MPP1 expression in rat lung extract (lane 1), mouse spleen extract (lane 2) and MCF-7 whole cell lysates (lane 3). MPP1 at 55KD was detected using rabbit anti- MPP1 Antigen Affinity purified polyclonal antibody (Catalog # PB1132) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).|Figure 2.ICC analysis using anti-MPP1 antibody (PB1132) was detected in immersion fixed MCF-7 cell line . Cells were stained using the Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) and counterstained with DAPI (blue).[/list_product_images]