手机验证
0
0
0
0
单克隆
human,monkey,rat
0
5-7天
博士德生物
WB,IHC-P,ELISA
0
200ul
产品名称 | AURKA Antibody |
---|---|
产品描述 | Anti-AURKA Antibody validated for IHC, WB, ELISA in Human, Monkey, Rat. |
文献引用格式 | AURKA Antibody (Boster Biological Technology, Wuhan, China. Catalog #BM3798) |
应用范围 | IHC, WB, ELISA |
抗体来源 | Mouse |
抗体亚型 | IgG1 |
免疫原 | Purified recombinant fragment of human AURKA expressed in E. Coli. |
抗体规格 | 200ul/1180元 |
克隆 | Monoclonal |
---|---|
克隆号 | 单克隆抗体:IgG1;克隆号:4G10 |
产品形态 | Liquid |
内容 | Ascitic fluid containing 0.03% sodium azide. |
储存条件 | 连续使用时4℃存储,保质期六个月;长期存储时建议分装为10ul以上小包装-20℃存储,并避免反复冻融,保质期一年。 |
小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
基因名 | AURKA |
---|---|
基因全名 | Aurora kinase A |
蛋白功能 | Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines. |
Tissue Specificity | Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis. . |
Sequence Similarities | Aurora kinase A;2.7.11.1;Aurora 2;Aurora/IPL1-related kinase 1;ARK-1;Aurora-related kinase 1;hARK1;Breast tumor-amplified kinase;Serine/threonine-protein kinase 15;Serine/threonine-protein kinase 6;Serine/threonine-protein kinase aurora-A;AURKA;AIK, AIRK1, ARK1, AURA, AYK1, BTAK, IAK1, STK15, STK6; |
Uniprot ID | AURKA: O14965 |
Aurora A plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment.Tissue specificity: Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
为了提供最优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
---|---|
Western blotting | 1:100-400 |
Immunohistochemistry in paraffin section | 1:50-200 |
Immunocytochemistry in fixed cells | 1:50-200 |
Flow Cytometry | 1:40-80 |
enzyme-linked immuno sorbent assay | 1:2000 |
*最优稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
点击图片放大
[list_product_images]AURKA(BM3798)(MW:46KD)HEK293,Sw620,MCF-7,Jurkat,Hela,HepG2, Cos7,PC-12细胞裂解,免疫印迹分析。|Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and lung cancer (right) using AURKA mouse mAb with DAB staining.|Red: Control Antigen (100ng);Purple: Antigen (10ng);Green: Antigen (50ng);Blue: Antigen (100ng);[/list_product_images]
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。