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Monoclonal
human
发货周期:5-7天
9999
博士德生物
WB, IHC, IF, FCM
50μl/100μl/150μl
规格: | 50μl | 产品价格: | ¥1060.0 |
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规格: | 100μl | 产品价格: | ¥1780.0 |
规格: | 150μl | 产品价格: | ¥2580.0 |
货号 | MA00001 |
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产品名称 | Anti-TP53 Antibody (Clone#OTI3D5) |
基因名 | TP53 |
抗体来源 | Mouse |
克隆 | OTI3D5 |
抗体亚型 | Mouse IgG1 |
分子量 | 43.5KD |
免疫原 | Full length human recombinant protein of human TP53 (NP_000537) produced in HEK293T cell. |
内容 | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
纯化方式 | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
浓度 | 500 ug/ml |
产品形态 | Liquid |
保存条件 | Stable for 12 months from date of receipt. Store at -20°C as received. |
背景资料 | This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants. |
研究类别 | 1. Aaltonen, L.-M., Chen, R. W., Roth, S., Makitie, A. A., Rihkanen, H., Vaheri, A., Aaltonen, L. A. Role of TP53 P72R polymorphism in human papillomavirus associated premalignant laryngeal neoplasm. J. Med. Genet. 38: 327 only, 2001. 2. Aas, T., Borresen, A.-L., Geisler, S., Smith-Sorenson, B., Johnsen, H., Varhaug, J. E., Akslen, L. A., Lonning, P. E. Specific P53 mutations are associated with de novo resistance to doxorubicin in breast cancer patients. Nature Med. 2: 811-814, 1996. 3. Abida, W. M., Nikolaev, A., Zhao, W., Zhang, W., Gu, W. FBXO11 promotes the neddylation of p53 and inhibits its transcriptional activity. J. Biol. Chem. 282: 1797-1804, 2007. |
Uniprot ID | TP53: P04637 |
博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
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Western blot (WB): | 1:500~2000 |
Immunohistochemistry in paraffin section (IHC): | 1:150 |
Immunofluorescence (IF): | 1:100 |
Flow cytometry (FCM): | 1:100 |
*最佳稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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