Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described. [provided by RefSeq, Jul 2013]
研究类别
1. Kariola, R., Raevaara, T. E., Lonnqvist, K. E., Nystrom-Lahti, M. Functional analysis of MSH6 mutations linked to kindreds with putative hereditary non-polyposis colorectal cancer syndrome. Hum. Molec. Genet. 11: 1303-1310, 2002. 2. Verma, L., Kane, M. F., Brassett, C., Schmeits, J., Evans, D. G. R., Kolodner, R. D., Maher, E. R. Mononucleotide microsatellite instability and germline MSH6 mutation analysis in early onset colorectal cancer. J. Med. Genet. 36: 678-682, 1999.
[list_product_images]Figure 1. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MSH6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MSH6 (Cat# MA00553)(1:2000).|Figure 2. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MSH6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA00553) (1:150)|Figure 3. Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and MSH6-Knockout hela cells (KO) were separated by SDS-PAGE and immunoblotted with anti-MSH6 monoclonal antibody MA00553. Then the blotted membrane was stripped and reprobed with anti-β-actin ([MA01263]) as a loading control (1:500).[/list_product_images]