产品概况
货号 |
MA00792 |
产品名称 |
Anti-CRy 2 Antibody (Clone#OTI1H5) |
基因名 |
CRY2 |
抗体来源 |
Mouse |
克隆 |
OTI1H5 |
抗体亚型 |
Mouse IgG2a |
分子量 |
66.8KD |
免疫原 |
Full length human recombinant protein of human CRY2 (NP_0066940) produced in HEK293T cell. |
内容 |
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
纯化方式 |
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
浓度 |
500 ug/ml |
产品形态 |
Liquid |
保存条件 |
Stable for 12 months from date of receipt. Store at -20°C as received. |
背景资料 |
This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. And it is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Two transcript variants encoding different isoforms have been found for this gene. |
研究类别 |
1. Kovanen L, Kaunisto M, Donner K, Saarikoski ST, Partonen T. “CRY2 genetic variants associate with dysthymia.” PLoS One, 2013.2. Mao Y, Fu A, Hoffman AE, Jacobs DI, Jin M, Chen K, Zhu Y. “The circadian gene CRY2 is associated with breast cancer aggressiveness possibly via epigenomic modifications.” Tumour Biol, 2015 May. |
Uniprot ID |
CRY2: Q49AN0 |
产品应用细节
博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 |
稀释度* |
Western blot (WB): |
1:2000 |
Immunohistochemistry in paraffin section (IHC): |
1:150 |
Immunofluorescence (IF): |
1:100 |
Flow cytometry (FCM): |
1:100 |
*最佳稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Flow cytometric Analysis of Hela cells, using anti-CRY2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).|Figure 2. Flow cytometric Analysis of Jurkat cells, using anti-CRY2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).|Figure 3. Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-CRY2 monoclonal antibody (1:200).|Figure 4. Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CRY2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00792)|Figure 5. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CRY2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00792)|Figure 6. Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CRY2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00792)|Figure 7. Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CRY2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00792)|Figure 8. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CRY2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00792)|Figure 9. Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CRY2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00792)|Figure 10. Immunofluorescent staining of HeLa cells using anti-CRY2 mouse monoclonal antibody.|Figure 11. Anti-CRY2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRY2 .|Figure 12. Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CRY2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00792)|Figure 13. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRY2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRY2(Cat# MA00792). [/list_product_images]