产品概况
货号 |
MA02163 |
产品名称 |
Anti-CELF1 Antibody (Clone#OTI5B8) |
基因名 |
CELF1 |
抗体来源 |
Mouse |
克隆 |
OTI5B8 |
抗体亚型 |
Mouse IgG2b |
分子量 |
52.0KD |
免疫原 |
Full length human recombinant protein of human CUGBP1 (NP_006551) produced in HEK293T cell. |
内容 |
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
纯化方式 |
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
浓度 |
500 ug/ml |
产品形态 |
Liquid |
保存条件 |
Stable for 12 months from date of receipt. Store at -20°C as received. |
背景资料 |
Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. This gene may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase (DMPK) gene. Alternative splicing results in multiple transcript variants encoding different isoforms. |
Uniprot ID |
CELF1: Q92879 |
产品应用细节
博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 |
稀释度* |
Western blot (WB): |
1:2000 |
Immunohistochemistry in paraffin section (IHC): |
1:50 |
Immunofluorescence (IF): |
1:100 |
*最佳稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-CUGBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02163, Dilution 1:50)|Figure 2. Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-CUGBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02163, Dilution 1:50)|Figure 3. Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-CUGBP1 monoclonal antibody (1:200).|Figure 4. Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-CUGBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02163, Dilution 1:50)|Figure 5. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-CUGBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02163, Dilution 1:50)|Figure 6. Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-CUGBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02163, Dilution 1:50)|Figure 7. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-CUGBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02163, Dilution 1:50)|Figure 8. Anti-CUGBP1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CUGBP1 .|Figure 9. Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-CUGBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02163, Dilution 1:50)|Figure 10. Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-CUGBP1 monoclonal antibody (1:200).|Figure 11. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CUGBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CUGBP1 (Cat# MA02163). [/list_product_images]