产品概况
货号 |
MA02460 |
产品名称 |
Anti-TLE1 Antibody (Clone#OTI1F5) |
基因名 |
TLE1 |
抗体来源 |
Mouse |
克隆 |
OTI1F5 |
抗体亚型 |
Mouse IgG2a |
分子量 |
83KD |
免疫原 |
Human recombinant protein fragment corresponding to amino acids 180-460 of human TLE1 (NP_005068) produced in E.coli. |
内容 |
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
纯化方式 |
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
浓度 |
500 ug/ml |
产品形态 |
Liquid |
保存条件 |
Stable for 12 months from date of receipt. Store at -20°C as received. |
背景资料 |
Enables identical protein binding activity and transcription corepressor activity. Involved in negative regulation of I-kappaB kinase/NF-kappaB signaling; negative regulation of anoikis; and regulation of gene expression. Located in cytosol and nucleoplasm. Part of beta-catenin-TCF complex. |
研究类别 |
1. Allen, T., van Tuyl, M., Iyengar, P., Jothy, S., Post, M., Tsao, M.-S., Lobe, C. G. Grg1 acts as a lung-specific oncogene in a transgenic mouse model. Cancer Res. 66: 1294-1301, 2006. 2. Cavallin, M., Maillard, C., Hully, M., Philbert, M., Boddaert, N., Reilly, M. L., Nitschke, P., Bery, A., Bahi-Buisson, N. TLE1, a key player in neurogenesis, a new candidate gene for autosomal recessive postnatal microcephaly. Europ. J. Med. Genet. 61: 729-732, 2018. 3. Gross, M. B. Personal Communication. Baltimore, Md. 1/3/2017. |
Uniprot ID |
TLE1: Q04724 |
产品应用细节
博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 |
稀释度* |
Western blot (WB): |
1:2000 |
Immunohistochemistry in paraffin section (IHC): |
1:150 |
Immunofluorescence (IF): |
1:100 |
Flow cytometry (FCM): |
1:100 |
*最佳稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TLE1 antibody, and then analyzed by flow cytometry.|Figure 2. Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02460)|Figure 3. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02460)|Figure 4. Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02460)|Figure 5. Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02460)|Figure 6. Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02460)|Figure 7. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02460)|Figure 8. Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02460)|Figure 9. Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02460)|Figure 10. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02460)|Figure 11. Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02460)|Figure 12. Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02460)|Figure 13. Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02460)|Figure 14. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02460)|Figure 15. Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02460)|Figure 16. Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02460)|Figure 17. Anti-TLE1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TLE1 .|Figure 18. Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02460)|Figure 19. Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02460)|Figure 20. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TLE1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TLE1(Cat# MA02460). [/list_product_images]