Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008]
[list_product_images]Figure 1. Flow cytometric analysis of living 293T cells transfected with LAG3 overexpression plasmid , Red)/empty vector (Blue) using anti-LAG3 antibody. Cells incubated with a non-specific antibody (Green) were used as isotype control. (1;100)|Figure 2. Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h (Right)/untreated (Left) using anti-LAG3 antibody (1:100).|Figure 3. Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h (Right) using anti-LAG3 antibody. Cells incubated with a non-specific antibody (Left) were used as isotype control (1:100).|Figure 4. Western blot analysis of extracts (35ug) from 2 cell lines (Human PBMC treated with 10ug/ml PHA for 72h (Right)/untreated (Left)) lysates by using anti-LAG3 monoclonal antibody (1:150).|Figure 5. Western blot analysis of extracts (10ug) from 1 human tissue by using anti-LAG3 monoclonal antibody at 1:200 dilution.|Figure 6. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAG3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LAG3. [/list_product_images]