产品概况
货号 |
MA05147 |
产品名称 |
Anti-ENDOG Antibody (Clone#OTI5F1) |
基因名 |
ENDOG |
抗体来源 |
Mouse |
克隆 |
OTI5F1 |
抗体亚型 |
Mouse IgG1 |
分子量 |
27.7KD |
免疫原 |
Full length human recombinant protein of human ENDOG(NP_004426) produced in HEK293T cell. |
内容 |
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
纯化方式 |
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
浓度 |
500 ug/ml |
产品形态 |
Liquid |
保存条件 |
Stable for 12 months from date of receipt. Store at -20°C as received. |
背景资料 |
The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA. [provided by RefSeq] |
Uniprot ID |
ENDOG: Q14249 |
产品应用细节
博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 |
稀释度* |
Western blot (WB): |
1:500~2000 |
Immunohistochemistry in paraffin section (IHC): |
1:150 |
Immunofluorescence (IF): |
1:100 |
Flow cytometry (FCM): |
1:100 |
*最佳稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ENDOG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA05147)|Figure 2. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ENDOG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA05147)|Figure 3. Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ENDOG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA05147)|Figure 4. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ENDOG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ENDOG(Cat# MA05147). |Figure 5. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ENDOG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA05147)|Figure 6. Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ENDOG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA05147)|Figure 7. Flow cytometric Analysis of Jurkat cells, using anti-ENDOG antibody, (Red), compared to a nonspecific negative control antibody, (Blue).|Figure 8. Flow cytometric Analysis of Hela cells, using anti-ENDOG antibody, (Red), compared to a nonspecific negative control antibody, (Blue).|Figure 9. Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ENDOG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA05147)|Figure 10. Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ENDOG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA05147)|Figure 11. Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ENDOG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA05147)|Figure 12. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ENDOG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA05147)|Figure 13. Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ENDOG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA05147)|Figure 14. Anti-ENDOG mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ENDOG .[/list_product_images]