产品概况
货号 |
MA05737 |
产品名称 |
Anti-GBP2 Antibody (Clone#OTI5C8) |
基因名 |
GBP2 |
抗体来源 |
Mouse |
克隆 |
OTI5C8 |
抗体亚型 |
Mouse IgG2b |
分子量 |
67KD |
免疫原 |
Full length human recombinant protein of human GBP2 (NP_004111) produced in HEK293T cell. |
内容 |
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
纯化方式 |
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
浓度 |
500 ug/ml |
产品形态 |
Liquid |
保存条件 |
Stable for 12 months from date of receipt. Store at -20°C as received. |
背景资料 |
Interferons are cytokines that have antiviral effects and inhibit tumor cell proliferation. They induce a large number of genes in their target cells, including those coding for the guanylate-binding proteins (GBPs). GBPs are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP). The protein encoded by this gene is a GTPase that converts GTP to GDP and GMP. [provided by RefSeq] |
研究类别 |
1. Cheng, Y.-S. E., Patterson, C. E., Staeheli, P. Interferon-induced guanylate-binding proteins lack an N(T)KXD consensus motif and bind GMP in addition to GDP and GTP. Molec. Cell. Biol. 11: 4717-4725, 1991. 2. Kim, B.-H., Shenoy, A. R., Kumar, P., Das, R., Tiwari, S., MacMicking, J. D. A family of IFN-gamma-inducible 65-kD GTPases protects against bacterial infection. Science 332: 717-721, 2011.3. Olszewski, M. A., Gray, J., Vestal, D. J. In silico genomic analysis of the human and murine guanylate-binding protein (GBP) gene clusters. J. Interferon Cytokine Res. 26: 328-352, 2006. |
Uniprot ID |
GBP2: Q9Z0E6 |
产品应用细节
博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 |
稀释度* |
Western blot (WB): |
1:2000 |
Immunohistochemistry in paraffin section (IHC): |
1:50 |
Immunofluorescence (IF): |
1:100 |
Flow cytometry (FCM): |
1:100 |
*最佳稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GBP2 antibody, and then analyzed by flow cytometry.|Figure 2. Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA05737)|Figure 3. Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA05737)|Figure 4. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GBP2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).|Figure 5. Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA05737)|Figure 6. Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA05737)|Figure 7. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA05737)|Figure 8. Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA05737)|Figure 9. Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA05737)|Figure 10. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA05737)|Figure 11. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA05737)|Figure 12. Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA05737)|Figure 13. Anti-GBP2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GBP2 .|Figure 14. Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA05737)|Figure 15. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBP2(Cat# MA05737). [/list_product_images]