产品概况
货号 |
MA06993 |
产品名称 |
Anti-TRAP alpha/TRAPA/SSR1 Antibody (Clone#OTI4H6) |
基因名 |
SSR1 |
抗体来源 |
Mouse |
克隆 |
OTI4H6 |
抗体亚型 |
Mouse IgG1 |
分子量 |
32.1KD |
免疫原 |
Full length human recombinant protein of human SSR1 (NP_003135) produced in HEK293T cell. |
内容 |
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
纯化方式 |
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
浓度 |
0.28 mg/ml |
产品形态 |
Liquid |
保存条件 |
Stable for 12 months from date of receipt. Store at -20°C as received. |
背景资料 |
The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. The SSR consists of 2 subunits, a 34-kD glycoprotein encoded by this gene and a 22-kD glycoprotein. This gene generates several mRNA species as a result of complex alternative polyadenylation. This gene is unusual in that it utilizes arrays of polyA signal sequences that are mostly non-canonical. [provided by RefSeq]. COMPLETENESS: complete on the 3' end. |
研究类别 |
1. Gross, M. B. Personal Communication. Baltimore, Md. 6/22/2012.2. Hartmann, E., Prehn, S. The N-terminal region of the alpha-subunit of the TRAP complex has a conserved cluster of negative charges. FEBS Lett. 349: 324-326, 1994. 3. Hirama, T., Miller, C. W., Koeffler, H. P. Translocon-associated protein alpha transcripts are induced by granulocyte-macrophage colony-stimulating factor and exhibit complex alternative polyadenylation. FEBS Lett. 455: 223-227, 1999. |
Uniprot ID |
SSR1: P43307 |
产品应用细节
博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 |
稀释度* |
Western blot (WB): |
1:2000 |
Immunohistochemistry in paraffin section (IHC): |
1:150 |
Immunofluorescence (IF): |
1:50~100 |
Flow cytometry (FCM): |
1:100 |
*最佳稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SSR1 antibody, and then analyzed by flow cytometry.|Figure 2. Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and SSR1-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-SSR1 monoclonal antibody MA06993 (1:2000`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.|Figure 3. Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA06993)|Figure 4. Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA06993)|Figure 5. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA06993)|Figure 6. Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-SSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA06993)|Figure 7. Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-SSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA06993)|Figure 8. Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA06993)|Figure 9. Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA06993)|Figure 10. Immunofluorescent staining of HeLa cells using anti-SSR1 mouse monoclonal antibody.|Figure 11. Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA06993)|Figure 12. Anti-SSR1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SSR1 .|Figure 13. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSR1. [/list_product_images]