产品概况
货号 |
MA07059 |
产品名称 |
Anti-UBE2J1/Ubc6E Antibody (Clone#OTI3C11) |
基因名 |
UBE2J1 |
抗体来源 |
Mouse |
克隆 |
OTI3C11 |
抗体亚型 |
Mouse IgG2a |
分子量 |
35KD |
免疫原 |
Ful length human recombinant protein of human UBE2J1(NP_057105) produced in HEK293T cell. |
内容 |
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
纯化方式 |
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
浓度 |
500 ug/ml |
产品形态 |
Liquid |
保存条件 |
Stable for 12 months from date of receipt. Store at -20°C as received. |
背景资料 |
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system. [provided by RefSeq, Jul 2008] |
Uniprot ID |
UBE2J1: Q9Y385 |
产品应用细节
博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 |
稀释度* |
Western blot (WB): |
1:2000 |
Immunohistochemistry in paraffin section (IHC): |
1:150 |
*最佳稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-UBE2J1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, MA07059)|Figure 2. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-UBE2J1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, MA07059)|Figure 3. Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-UBE2J1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, MA07059)|Figure 4. Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-UBE2J1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, MA07059)|Figure 5. Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-UBE2J1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, MA07059)|Figure 6. Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-UBE2J1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, MA07059)|Figure 7. Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-UBE2J1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, MA07059)|Figure 8. Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-UBE2J1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, MA07059)|Figure 9. Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-UBE2J1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, MA07059)|Figure 10. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2J1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2J1. [/list_product_images]