E.coli-derived human C19orf2/URI1 recombinant protein (Position: K89-Q532).
内容
500 ug/ml antibody with PBS,0.02% NaN3, 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Unconventional prefoldin RPB5 interactor, also called URI1, is a protein that in humans is encoded by the URI1 gene. This gene encodes member of the prefoldin family of molecular chaperones. The encoded protein functions as a scaffolding protein and plays roles in ubiquitination and transcription, in part though interactions with the RNA polymerase II subunit RPB5. This gene may play a role in multiple malignancies including ovarian cancer and hepatocellular carcinoma. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 22.
[list_product_images]Figure 1. Western blot analysis of anti-C19orf2/URI1 antibody (A05245-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Daudi whole cell lysates,
Lane 4: human HEL1 whole cell lysates,
Lane 5: mouse ovary tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-C19orf2/URI1 antigen affinity purified polyclonal antibody (A05245-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for C19orf2/URI1 at approximately 80 kDa. The expected band size for C19orf2/URI1 is at 60 kDa.|Figure 2. Flow Cytometry analysis of RT4 cells using anti-C19orf2/URI1 antibody (A05245-2).
Overlay histogram showing RT4 cells stained with A05245-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C19orf2/URI1 Antibody (A05245-2, 1 μg/1x106 cells). DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]