大家都在搜
手机验证
Polyclonal
human, mouse, rat
发货周期:现货
9999
博士德生物
WB, ICC/IF, FCM(Intracellular), Direct ELISA
50μl/100μl/150μl
规格: | 50μl | 产品价格: | ¥1060.0 |
---|---|---|---|
规格: | 100μl | 产品价格: | ¥1780.0 |
规格: | 150μl | 产品价格: | ¥2580.0 |
货号 | A09003-1 |
---|---|
产品名称 | Anti-NDUFA5 Antibody |
基因名 | NDUFA5 |
抗体来源 | Rabbit |
抗体亚型 | Rabbit IgG |
分子量 | 16KD |
免疫原 | E.coli-derived human NDUFA5 recombinant protein (Position: M1-I116). |
内容 | 500 ug/ml antibody with PBS,0.02% NaN3, 1 mg BSA and 50% glycerol. |
纯化方式 | Immunogen affinity purified. |
浓度 | 500 ug/ml |
产品形态 | Liquid |
保存条件 | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
背景资料 | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 is an enzyme that in humans is encoded by the NDUFA5 gene. This nuclear gene encodes a conserved protein that comprises the B13 subunit of complex I of the mitochondrial respiratory chain. The encoded protein localizes to the inner mitochondrial membrane, where it is thought to aid in the transfer of electrons from NADH to ubiquinone. Alternative splicing results in multiple transcript variants. There are numerous pseudogenes of this gene on chromosomes 1, 3, 6, 8, 9, 11, 12, and 16. |
研究类别 | 1. Pata, I., Tensing, K., Metspalu, A. A human cDNA encoding the homologue of NADH:ubiquinone oxidoreductase subunit B13. Biochim. Biophys. Acta 1350: 115-118, 1997. 2. Peralta, S., Torraco, A., Wenz, T., Garcia, S., Diaz, F., Moraes, C. T. Partial complex I deficiency due to the CNS conditional ablation of Ndufa5 results in a mild chronic encephalopathy but no increase in oxidative damage. Hum. Molec. Genet. 23: 1399-1412, 2014. 3. Russell, M. W., du Manoir, S., Collins, F. S., Brody, L. C. Cloning of the human NADH:ubiquinone oxidoreductase subunit B13: localization to chromosome 7q32 and identification of a pseudogene on 11p15. Mammalian Genome 8: 60-61, 1997. |
Uniprot ID | NDUFA5: Q16718 |
推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for ICC. |
博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
---|---|
Western blot (WB): | 1:500-2000 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
Flow Cytometry(Intracellular): | 1-3 μg/1x106 cells |
Direct ELISA: | 1:100-1000 |
*最佳稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。