E.coli-derived human NT5C recombinant protein (Position: H35-R179).
内容
500 ug/ml antibody with PBS,0.02% NaN3, 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
5', 3'-nucleotidase, cytosolic, also known as 5'(3')-deoxyribonucleotidase, cytosolic type (cdN) or deoxy-5'-nucleotidase 1 (dNT-1), is an enzyme that in humans is encoded by the NT5C gene on chromosome 17. This gene encodes a nucleotidase that catalyzes the dephosphorylation of the 5' deoxyribonucleotides (dNTP) and 2'(3')-dNTP and ribonucleotides, but not 5' ribonucleotides. Of the different forms of nucleotidases characterized, this enzyme is unique in its preference for 5'-dNTP. It may be one of the enzymes involved in regulating the size of dNTP pools in cells. Alternatively spliced transcript variants have been found for this gene.
研究类别
1. Anderson, J. A., Teng, Y.-S., Giblett, E. R. Stains for six enzymes potentially applicable to chromosomal assignment by cell hybridization. Cytogenet. Cell Genet. 14: 295-299, 1975.2. Hoglund, L., Reichard, P. Cytoplasmic 5-prime(3-prime)-nucleotidase from human placenta. J. Biol. Chem. 265: 6589-6595, 1990. 3. Paglia, D. E., Valentine, W. N., Brockway, R. A. Identification of thymidine nucleotidase and deoxyribonucleotidase activities among normal isozymes of 5-prime-nucleotidase in human erythrocytes. Proc. Nat. Acad. Sci. 81: 588-592, 1984.
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.