手机验证
0
0
0
0
单克隆
human,mouse
0
5-7天
博士德生物
WB,IHC-P,FC,ELISA
0
200ul
产品名称 | E2F1 Antibody |
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产品描述 | Anti-E2F1 Antibody validated for IHC, WB, FC, ELISA in Human, Mouse. |
文献引用格式 | E2F1 Antibody (Boster Biological Technology, Wuhan, China. Catalog #BM0597) |
应用范围 | IHC, WB, FC, ELISA |
抗体来源 | Mouse |
抗体亚型 | IgG1 |
免疫原 | Purified recombinant fragment of human E2F1 (AA: 69-223) expressed in E. Coli. |
抗体规格 | 200ul/1180元 |
克隆 | Monoclonal |
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克隆号 | 单克隆抗体:IgG1;克隆号:8G9 |
产品形态 | Liquid |
内容 | Ascitic fluid containing 0.03% sodium azide. |
储存条件 | 连续使用时4℃存储,保质期六个月;长期存储时建议分装为10ul以上小包装-20℃存储,并避免反复冻融,保质期一年。 |
小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
基因名 | E2F1 |
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基因全名 | Transcription factor E2F1 |
Tissue Specificity | Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812). . |
Sequence Similarities | Transcription factor E2F1;E2F-1;PBR3;Retinoblastoma-associated protein 1;RBAP-1;Retinoblastoma-binding protein 3;RBBP-3;pRB-binding protein E2F-1;E2F1;RBBP3; |
Uniprot ID | E2F1: Q01094 |
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
为了提供最优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
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Western blotting | 1:100-400 |
Immunohistochemistry in paraffin section | 1:50-200 |
Immunocytochemistry in fixed cells | 1:50-200 |
Flow Cytometry | 1:40-80 |
enzyme-linked immuno sorbent assay | 1:2000 |
*最优稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Western blot analysis using E2F1 mAb against human E2F1 recombinant protein. (Expected MW is 42.7 kDa) |Western blot analysis using E2F1 mouse mAb against HeLa (1), SK-N-SH (2), and NIH3T3 (3) cell lysate. |Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using E2F1 mouse mAb with DAB staining. |Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using E2F1 mouse mAb with DAB staining. |Flow cytometric analysis of HeLa cells using E2F1 mouse mAb (green) and negative control (red). |Control Antigen (100 ng);Purple line: Antigen(10ng);Blue line: Antigen (50 ng);Red line: Antigen (100 ng);[/list_product_images]
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