E.coli-derived human FOXRED2 recombinant protein (Position: Q44-Q623).
内容
500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
FOXRED2, also known as ERFAD, is an endoplasmic reticulum (ER) luminal flavoprotein which may function in ER associated degradation (ERAD). It has been proposed to bind non-native proteins in the endoplasmic reticulum and target them to the ubiquitination machinery for subsequent degradation. Upon knockdown of FOXRED2, the degradation of the ERAD model substrate ribophorin 332 is delayed, and the overall level of polyubiquitinated cellular proteins is decreased. Defects in the related protein FOXRED1 are a cause of mitochondrial complex I deficiency (MT-C1D), suggesting the FOXRED2 may also play a role in MT-C1D.
研究类别
1. Hartz, P. A. Personal Communication. Baltimore, Md. 2/25/2011.2. Riemer, J., Appenzeller-Herzog, C., Johansson, L., Bodenmiller, B., Hartmann-Petersen, R., Ellgaard, L. A luminal flavoprotein in endoplasmic reticulum-associated degradation. Proc. Nat. Acad. Sci. 106: 14831-14836, 2009.
[list_product_images]Figure 1. Western blot analysis of anti- FOXRED2 Antibody (A12703-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: HepG2 whole cell lysates,
Lane 3: Jurkat whole cell lysates,
Lane 4: C6 whole cell lysates,
Lane 5: NIH/3T3 whole cell lysates.
Use rabbit anti- FOXRED2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for FOXRED2 at approximately 80KD. The expected band size for FOXRED2 is at 78KD.[/list_product_images]