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A synthetic peptide corresponding to a sequence at the C-terminus of mouse AKT1 (397-421aa EDAKEIMQHRFFANIVWQDVYEKKL), different from the related human sequence by two amino acids, and identical to the related rat sequence.
Rabbit IgG
Lyophilized
12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.
单克隆
human,mouse,rat
Rabbit
现货
博士德生物
WB
500ug/ml
50ul/100ul/150ul
规格: | 50ul | 产品价格: | ¥860.0 |
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规格: | 100ul | 产品价格: | ¥1520.0 |
规格: | 150ul | 产品价格: | ¥2280.0 |
货号 | A00024 |
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产品名称 | ANTI-AKT1 Antibody |
基因名 | Akt1 |
抗体来源 | Rabbit |
克隆 | Polyclonal |
抗体亚型 | Rabbit IgG |
分子量 | 55KD |
免疫原 | A synthetic peptide corresponding to a sequence at the C-terminus of mouse AKT1 (397-421aa EDAKEIMQHRFFANIVWQDVYEKKL), different from the related human sequence by two amino acids, and identical to the related rat sequence. |
内容 | 500ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA |
纯化方式 | Immunogen affinity purified. |
浓度 | 500ug/ml |
产品形态 | Lyophilized |
保存条件 | 12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
背景资料 | RAC-alpha serine/threonine-protein kinase is an enzyme that in humans is encoded by the AKT1 gene. The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. |
研究类别 | 1. "Entrez Gene: AKT1 v-akt murine thymoma viral oncogene homolog 1".2. Lindhurst MJ, Sapp JC, Teer JK, Johnston JJ, Finn EM, Peters K, Turner J, Cannons JL, Bick D, Blakemore L, Blumhorst C, Brockmann K, Calder P, Cherman N, Deardorff MA, Everman DB, Golas G, Greenstein RM, Kato BM, Keppler-Noreuil KM, Kuznetsov SA, Miyamoto RT, Newman K, Ng D, O'Brien K, Rothenberg S, Schwartzentruber DJ, Singhal V, Tirabosco R, Upton J, Wientroub S, Zackai EH, Hoag K, Whitewood-Neal T, Robey PG, Schwartzberg PL, Darling TN, Tosi LL, Mullikin JC, Biesecker LG (2011). "A mosaic activating mutation in AKT1 associated with the Proteus syndrome". N. Engl. J. Med. 365 (7): 611–9. |
Uniprot ID | Akt1: P31750 |
推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
为了提供最优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
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Western blot: | 1:500-2000 |
*最优稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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