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Polyclonal
human, monkey, mouse, rat
发货周期:现货
9999
博士德生物
WB, ICC/IF, FCM
50μl/100μl/150μl
规格: | 50μl | 产品价格: | ¥1060.0 |
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规格: | 100μl | 产品价格: | ¥1780.0 |
规格: | 150μl | 产品价格: | ¥2580.0 |
货号 | A08652-1 |
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产品名称 | Anti-ACADSB Antibody |
基因名 | ACADSB |
抗体来源 | Rabbit |
克隆 | Polyclonal |
抗体亚型 | Rabbit IgG |
分子量 | 40-44KD |
免疫原 | A synthetic peptide corresponding to a sequence of human SBCAD/ACADSB (ASNIQLNTIAKHIDAEY). |
内容 | 500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol. |
纯化方式 | Immunogen affinity purified. |
浓度 | 500 ug/ml |
产品形态 | Liquid |
保存条件 | 12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
背景资料 | ACADSB is a human gene that encodes short/branched chain specific acyl-CoA dehydrogenase (SBCAD), an enzyme in the acyl CoA dehydrogenase family.Short/branched chain acyl-CoA dehydrogenase(ACADSB) is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. Substrate specificity is the primary characteristic used to define members of this gene family. The ACADSB gene product has the greatest activity towards the short branched chain acyl-CoA derivative, (S)-2-methylbutyryl-CoA, but also reacts significantly with other 2-methyl branched chain substrates and with short straight chain acyl-CoAs. The cDNA encodes for a mitochondrial precursor protein which is cleaved upon mitochondrial import and predicted to yield a mature peptide of approximately 43.7-KDa. |
研究类别 | 1. Alfardan, J., Mohsen, A.-W., Copeland, S., Ellison, J., Keppen-Davis, L., Rohrbach, M., Powell, B. R., Gillis, J., Matern, D., Kant, J., Vockley, J. Characterization of new ACADSB gene sequence mutations and clinical implications in patients with 2-methylbutyrylglycinuria identified by newborn screening. Molec. Genet. Metab. 100: 333-338, 2010.2. Andresen, B. S., Christensen, E., Corydon, T. J., Bross, P., Pilgaard, B., Wanders, R. J. A., Ruiter, J. P. N., Simonsen, H., Winter, V., Knudsen, I., Schroeder, L. D., Gregersen, N., Skovby, F. Isolated 2-methylbutyrylglycinuria caused by short/branched-chain acyl-CoA dehydrogenase deficiency: identification of a new enzyme defect, resolution of its molecular basis, and evidence for distinct acyl-CoA dehydrogenases in isoleucine and valine metabolism. Am. J. Hum. Genet. 67: 1095-1103, 2000.3. Arden, K. C., Viars, C. S., Fu, K., Rozen, R. Localization of short/branched chain acyl-CoA dehydrogenase (ACADSB) to human chromosome 10. Genomics 25: 743-745, 1995. |
Uniprot ID | ACADSB: P45954 |
推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for ICC. |
博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
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Western blot(WB): | 1:500-2000 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
Flow cytometry (FCM): | 1-3 μg/1x106 cells |
*最佳稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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