ScreeNingBio CHO-K1 Rat FFA1 (GPR40) Gq Cell Line Background CHO-K1 Rat FFA1 (GPR40) Gq cell line overexpress rat FFAR1 and is designed to detect increases in intracellular IP-1 levels in response to agonist stimulation of the receptor. Cisbio HTRF IP-1 kit can be used to detect the signal. Product Information Catalog Number:SNB-G-0093C Lot Number:See vial Contents:2 vials, 1 x 106 cells per vial Target GPCR:free fatty acid receptor 1 (FFA1) HGNC Symbol: FFAR1 Coupling:Gq Accession No.:NM_153304 GPCR Species:Rat Host Cell:CHO-K1 Culture Medium:F12 + 10% FBS Antibiotics:200 μg/mL Hygromycin B Storage:Store in vapor phase of liquid nitrogen upon receiving Functional Assay Data In a 384-well plate, cells were stimulated with agonist compounds using the assay conditions described below. Following stimulation, signal was detected using the Cisbio HTRF IP-1 assay detection kit according to the recommended protocol.
Agonist EC50 was 0.94 nM and signal window 3.5 fold.
Passage Stability
This cell line has been confirmed to be stable through 10 passages (4 weeks) with no significant drop in assay window or change in EC50 of control compound.
Price Information ¥79,999 for 2 vial of frozen cell ¥7,999 for one 96 well compound testing (4 compounds, 10 point, ½ log dilution in duplicate) ¥11,999 for one 384 well compound testing (16 compounds, 10 point, ½ log dilution in duplicate) For additional information, please visit www.screeningbio.com or info@screeningbio.com 上海格宁生物科技有限公司