WB result of CD7 Rabbit mAb Primary antibody: CD7 Rabbit mAb at 1/500 dilution Lane 1: Raji whole cell lysate 20ug Lane 2: Jurkat whole cell lysate 20ug Negative control: Raji whole cell lysate Secondary antibody: #abs20040 at 1/10000 dilution Predicted MW: 25.4kDa Observed MW: 37 kDa Exposure time: 90s
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD7 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-CD7 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human NK/T-cell lymphoma. Anti-CD7 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ICC shows positive staining in Jurkat cells. Anti-CD7 antibody was used at 1/125 dilution (Green) and incubated overnight at 4°C. Secondary antibody: #abs20025 at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Negative control: ICC shows negative staining in Raji cells. Anti-CD7 antibody was used at 1/125 dilution and incubated overnight at 4°C. Secondary antibody: #abs20025 at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).