- ￥1190 - 1960
- AntibodySystem
- France
- FHC13140
- 2025年07月15日
- FCM, WB
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2a
- 形态：
Liquid
- 保存条件：
-20 to -80°C
- 克隆性：
单克隆
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
twelve months
- 级别：
流式抗体
- 供应商：
武汉益普生物科技有限公司
- 宿主：
Mouse
- 应用范围：
FCM, WB
- 抗体英文名：
Anti-Human CD18/ITGB2 Antibody (60.3)
- 抗体名：
CD18/ITGB2抗体
- 规格：
50μg/100μg
| 规格： | 50μg | 产品价格： | ￥1190.0 |
|---|---|---|---|
| 规格： | 100μg | 产品价格： | ￥1960.0 |
| 产品名 | Anti-Human CD18/ITGB2 Antibody (60.3) |
| 货号 | FHC13140 |
| 靶标 | Complement receptor C3 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta, Integrin beta-2, MFI7, CD18, ITGB2 |
| 克隆号 | 60.3 |
| 克隆类型 | Monoclonal |
| 同种型 | IgG2a |
| 宿主 | Mouse |
| 种属反应性 | Human |
| 应用 | FCM, WB |
| 偶联物 | Unconjugated |
| 内毒素水平 | Please contact with the lab for this information. |
| 纯度 | >95% as determined by SDS-PAGE. |
| 纯化方式 | Protein A/G purified from cell culture supernatant. |
| 状态 | Liquid |
| 保存溶液 | 0.01M PBS, pH 7.4, 0.09% Sodium azide. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Note | For research use only. |
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文献和实验1. Treat cells. For PC12 or NIH 3T3 cells a moderately confluent 60 mm plate is used. 2. Aspirate media. Rinse cells with PBS, aspirate.3. Lyse cells in 200 uL of SDS sample buffer (100 ℃). Immediately scrape the cells off the plate with a rubber
% nonfat dry milk works well for anti-PCREB, but in my hands milk is not as good as BSA from USB (it seems to mask the epitope). Remove block solution and add primary antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000
ELISA Procedure for Measuring Serum Antibody Titer
of an antibody is shown in Figure 22. The titer was estimated to be 1 in 2400. Figure 22. Antibody titer graph (X = dilution corresponding to Ab titer) Further Methods for Evaluating Anti-peptide Antibodies If the antibodies are to be used as a reagent
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