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- 详细信息
- 技术资料
- 保存条件:
Store at -20ºC or below. After reconstitution, keep as concentrated solution. Aliquot and avoid freeze-thaw cycles.
- 保质期:
12 months from the shipping date of the product.
- 英文名:
Rat Progranulins protein, DDDDK tag
- 库存:
Available
- 供应商:
GeneTex
- 规格:
10 μg
Deglycosylation of GTX65638-pro rat progranulin (DDDDK tagged) protein. To examine the deglycosylation of rat Progranulin (DDDDDK tagged), 1 μg of rat progranulin is denatured with 1X glycoprotein denaturing buffer at 100ºC for 10 minutes. After the addition of NP-40 and G7 reaction buffer, twofold dilutions of PNGase F are added and the reaction mix is incubated for 1 or 3 hours at 37ºC. Separation of reaction products is visualized by immunoblotting using anti-DDDDDK-HRP antibody.
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