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p70 S6K (phospho Thr421/Ser424

) antibody [HL129]
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  • ¥1700 - 4000
  • GeneTex已认证
  • 美国
  • GTX635621
  • 2025年07月09日
  • WB, IHC-P
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Carrier-protein conjugated synthetic peptide surrounding phospho Thr421/Ser424 of human p70 S6K. The exact sequence is proprietary.

    • 亚型

      IgG

    • 形态

      Liquid

    • 保存条件

      Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

    • 克隆性

      Monoclonal

    • 标记物

      Unconjugated

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      12 months from the shipping date of the product. 

    • 抗原来源

      Human

    • 目录编号

      GTX635621

    • 级别

      Primary Antibodies

    • 库存

      Available

    • 供应商

      GeneTex

    • 宿主

      Rabbit

    • 应用范围

      WB, IHC-P

    • 浓度

      1 mg/ml (Please refer to the vial label for the specific concentration.)

    • 靶点

      p70 S6K (phospho Thr421/Ser424)

    • 抗体英文名

      p70 S6K (phospho Thr421/Ser424) antibody [HL129]

    • 抗体名

      p70 S6K (phospho Thr421/Ser424) 抗体 [HL129]

    • 规格

      100 μl/25 μl

    规格:100 μl产品价格:¥4000.0
    规格:25 μl产品价格:¥1700.0
    产品细节图片1

    Wild-type (WT) and p70 S6K (phospho Thr421/Ser424) knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p70 S6K (phospho Thr421/Ser424) antibody [HL129] (GTX635621) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

    p70 S6K (phospho Thr421/Ser424) antibody [HL5] detects p70 S6K (phospho Thr421/Ser424) protein at cytoplasm by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right).
    Sample: Paraffin-embedded mouse intestine.
    p70 S6K (phospho Thr421/Ser424) stained by p70 S6K (phospho Thr421/Ser424) antibody [HL5] (GTX635621) diluted at 1:100.
    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

    产品细节图片2

    Untreated (–) and treated (+) Rat2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p70 S6K (phospho Thr421/Ser424) antibody [HL129] (GTX635621) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

    产品细节图片3

    p70 S6K (phospho Thr421/Ser424) antibody [HL5] detects p70 S6K (phospho Thr421/Ser424) protein by immunohistochemical analysis.
    Sample: Paraffin-embedded rat pancreas.
    p70 S6K (phospho Thr421/Ser424) stained by p70 S6K (phospho Thr421/Ser424) antibody [HL5] (GTX635621) diluted at 1:100.
    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

    产品细节图片4

    p70 S6K (phospho Thr421/Ser424) antibody [HL5] detects p70 S6K (phospho Thr421/Ser424) protein by immunohistochemical analysis.
    Sample: Paraffin-embedded mouse tissue.
    p70 S6K (phospho Thr421/Ser424) stained by p70 S6K (phospho Thr421/Ser424) antibody [HL5] (GTX635621) diluted at 1:100.
    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

    产品细节图片5

    Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with p70 S6K (phospho Thr421/Ser424) antibody [HL129] (GTX635621) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

    *The competitor is not affiliated with GeneTex and does not endorse this product.

    产品细节图片6

    Untreated (–) and treated (+) Rat2 whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with p70 S6K (phospho Thr421/Ser424) antibody [HL129] (GTX635621) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

    *The competitor is not affiliated with GeneTex and does not endorse this product.

    产品细节图片7

    Untreated (–) and treated (+) Neuro2A whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with p70 S6K (phospho Thr421/Ser424) antibody [HL5] (GTX635621) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

    *The competitor is not affiliated with GeneTex and does not endorse this product.

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    图标文献和实验
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    • v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平

      4 ). 2. Other antibodies used were b-catenin (phospho-Thr41/Ser37/Ser33) (#9561; Cell Signaling Technology), b-catenin (#9562;Cell Signaling Technology), CRMP2 (pT514/509) (#PB-043; Kinasource, Dundee, Scotland) and CRMP2 (#AB-042;Kinasource). 3. Blocking buffer

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