- ￥5600
- GeneTex
- 美国
- GTX64104
- 2025年07月11日
- WB
- Rabbit
- Schizosaccharomyces pombe
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant GST-fusion protein with the N-terminal half of Cut5 protein
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Schizosaccharomyces pombe
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Yeast
- 目录编号：
GTX64104
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
Reacts with S. pombe Cut5/Rad4 protein. Not tested for other species
- 抗体英文名：
Cut5 / Rad4 (S. pombe) antibody
- 抗体名：
Cut5 / Rad4 (S. pombe) 抗体
- 规格：
100 μl
Identification of the Cut5/Rad4 protein in the crude extract of S. pombe with this antibody. Samples were prepared by alkali-lysis of the cells followed by TCA precipitation of proteins. Lane M: Size markers (kDa) Lane 1: Wild-type cells Lane 2: The cut5-5Flag gene replacing the wild-type cut5 gene Lane 3: The cut5-13myc gene replacing the wild type gene Lane 4: The cut-TAP gene replacing the wild-type gene * Cut5 protein is known to be sensitive for protease digestion in the C-terminal region.
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文献和实验Kamps's Western Blotting Protocol
in immunoprecipitates than anti-immunoglobulin antibodies.ECL analysis of immunoprecipitates using a second antibody is almost always blighted by strong staining of the antibody bands and by the staining of abundant proteins in the sample.Blots developed with iodinated
monoclonal antibody production fusion
SOLUTIONS IMDM (Iscove's Modified Dulbecco's Medium with 25 mM Hepes/with L-Glutamine) BioWhittaker Europe Cat.: BE12-722F IMDM-FCS(10%v/v)-HTx100(1%v/v)-AJ(5%v/v)-IL-6 (100 U/ml) IMDM - FCS(10%v/v) - HTx
Purification Of Anti Peptide Antibody
a parastaltic pump and recirculate the serum ON or just batch bind the serum ON. Wash with 5 col vols TBS. Wash with 10 col vols 0.5 M NaCl, 20mM TrisCl pH 7.4, 0.2 Triton- X-100. Wash 5 col vols TBS Elute with 0.15 M NaCl, 0.2 M Glycine
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