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Tfa1 / TFIIEa (S. cerevisiae)

antibody
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  • ¥5600
  • GeneTex已认证
  • 美国
  • GTX64183
  • 2025年07月09日
  • WB
  • Rabbit
  • Saccharomyces cerevisiae
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Recombinant His-tagged Tfa1 protein (1-482 aa) produced in E. coli

    • 亚型

      IgG

    • 形态

      Liquid

    • 保存条件

      Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

    • 克隆性

      Polyclonal

    • 标记物

      Unconjugated

    • 适应物种

      Saccharomyces cerevisiae

    • 保质期

      12 months from the shipping date of the product. 

    • 目录编号

      GTX64183

    • 级别

      Primary Antibodies

    • 库存

      Available

    • 供应商

      GeneTex

    • 宿主

      Rabbit

    • 应用范围

      WB

    • 浓度

      Batch dependent (Please refer to the vial label for the specific concentration.)

    • 靶点

      Tfa1 / TFIIEa (S. cerevisiae)

    • 抗体英文名

      Tfa1 / TFIIEa (S. cerevisiae) antibody

    • 抗体名

      Tfa1 / TFIIEa (S. cerevisiae) 抗体

    • 规格

      100 μl

    产品细节图片1

    Detection of endonenous Tfa1 in whole cell extract of S. cerevisiae by Western blotting, using the anti-Tfa1 antibody The antibody was used at 1/2,000 dilution. As second antibody, HRP-conjugated goat anti-rabbit IgG was used at 1/10,000 The apparent molecular mass (~65 kDa) is larger than calculated mass of 55 kDa, which may be due to its highly acidic cluster at C-terminal 106 amino-acids (Asp/Glu rich).

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    图标文献和实验
    相关实验
    • Chromatin immunoprecipitation protocol for Saccharomyces cerevisiae

      double-strand breaks (DSBs) in budding yeast (Saccharomyces cerevisiae ) (Cobb et al., 2003; van Attikum et al., 2004). Back to top Procedure Preculture of yeast cells for ChIP of proteins at DSBs We have used strains with JKM179

    • Kamps's Western Blotting Protocol

      in immunoprecipitates than anti-immunoglobulin antibodies.ECL analysis of immunoprecipitates using a second antibody is almost always blighted by strong staining of the antibody bands and by the staining of abundant proteins in the sample.Blots developed with iodinated

    • monoclonal antibody production fusion

      SOLUTIONS IMDM (Iscove's Modified Dulbecco's Medium with 25 mM Hepes/with L-Glutamine) BioWhittaker Europe Cat.: BE12-722F IMDM-FCS(10%v/v)-HTx100(1%v/v)-AJ(5%v/v)-IL-6 (100 U/ml) IMDM - FCS(10%v/v) - HTx

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