Synthetic phosphopeptide derived from the region of Insulin Receptor that contains tyrosines 1150 and 1151. The corresponding residues in the IGF1R are 1135 and 1136.
亚型:
IgG
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
克隆性:
Polyclonal
标记物:
Unconjugated
适应物种:
Human, Mouse, Rat
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX25680
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Rabbit
应用范围:
WB, ICC/IF, IHC-P, FACS, IHC
浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
ICC/IF analysis of MCF7 cells treated with insulin (100nM for 5 min) using GTX25680 IGF1R (phospho Tyr1135 / Tyr1136) / Insulin Receptor (phospho Tyr1150 / Tyr1151) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% PFA Permeabilization : 0.25% Triton X-100 for 10 minutes
WB (peptide competition) analysis of CHO-T cells over-expressing the human insulin receptor stimulated with 100 nM insulin for 10 min at 37ºC (Lane 2-5) using GTX25680 IGF1R (phospho Tyr1135 / Tyr1136) / Insulin Receptor (phospho Tyr1150 / Tyr1151) antibody prior incubated with the phosphopeptide immunogen (Lane 2), the non-phosphorylated peptide corresponding to the phosphopeptide immunogen (Lane 3), or a generic phosphotyrosine-containing peptide (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.