- ￥5600
- GeneTex
- 美国
- GTX64145
- 2025年07月14日
- WB, ICC/IF, IP
- Rabbit
- Schizosaccharomyces pombe
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant C-terminal polypeptide (26kDa) of S. pombe Ppa2
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Schizosaccharomyces pombe
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Yeast
- 目录编号：
GTX64145
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IP
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
The antibody recognized both Ppa1 and Ppa2 polypeptides in S. pombe because of their high amino acid similarity (~80% identity)
- 抗体英文名：
Ppa2 (S. pombe) antibody
- 抗体名：
Ppa2 (S. pombe) 抗体
- 规格：
100 μl
Cellular location of Ppa1 and Ppa2. Indirect immunofluorescence microscopy of ppa2 deletion, wild type (WT), and wild-type overexpressing ppa2 (nmt-ppa2, 18hr) was done, using anti-ppa2 antibody (right); The same cells stained by DAPI are also shown (left). Immunofluorescence was hardly detected in ppa2 cells, whereas cytoplasmic immunofluorescence was abundant in wild-type cells. Wild-type cells carrying nmt-ppa2 plasmid overexpress Ppa2 protein in the absence of thiamine for 18 hr. Immunofluorescence was enhanced further in the cytoplasm, often accumulated at the nuclear periphery or within restricted domains. The deformation of chromosomal DNA was also visible in overexpressed cells. Bar, 10um
Identification of Ppa1 and Ppa2 proteins. An immunoblot with anti-ppa2 antibody is shown (ref.1). lane 1: Wild-type S. pombe lane 2: ppa1 lane 3: ppa2 lane 4: Wild-type carrying a multicopy plasmid with ppa1 gene lane 5: Wild-type carrying a multicopy plasmid with ppa2 gene lane 6:Wild-type carrying a multicopy plasmid with ADH promoter ligated with ppa2 gene The positions of ppa1 (36 kDa) and ppa2 (39 kDa) polypeptide bands are indicated.
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文献和实验Chimerization of a Monoclonal Antibody for Treating Hodgkin's Lymphoma
follicles and at the rim of germinal centers (2 ). Consequently, Ber-H2 has been utilized as an immunotoxin by conjugating the antibody to the ribosome-inactivating toxin Saporin to treat four patients with refractory Hodgkin’s lymphoma
protein (GFP) under the control of an attenuated nmt1 promoter in Schizosaccharomyces pombe . The antibody binds to its target antigen without inhibiting protein function, allowing visualization of its intracellular location in fixed or living cells.
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
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