Recombinant protein corresponding to SARS-CoV-2 (COVID-19) Spike RBD (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.
亚型:
IgG
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
靶点:
This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on our internal testing.
抗体英文名:
SARS-CoV-2 (COVID-19) Spike RBD antibody
抗体名:
SARS-CoV-2 (COVID-19) Spike RBD 抗体
规格:
100 μl/25 μl
规格:
100 μl
产品价格:
¥4000.0
规格:
25 μl
产品价格:
¥1700.0
Non-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) detects SARS-CoV-2 (COVID-19) Spike RBD protein by flow cytometry analysis. Sample: 293T cells transfected SARS-CoV-2 (2019-nCoV) Spike. Blue: Unlabelled sample was used as a control. Red: SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.
Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
Indirect ELISA analysis performed by coating plate with recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.529 Omicron variant) (159.36-2.49 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
SARS-CoV-2 (COVID-19) Spike RBD antibody detects SARS-CoV-2 (COVID-19) Spike RBD protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% PFA at RT for 15 min.Green: SARS-CoV-2 (COVID-19) Spike RBD stained by SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
SARS-CoV-2 (COVID-19) Spike RBD antibody detects SARS-CoV-2 (COVID-19) Spike RBD protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640).Green: SARS-CoV-2 (COVID-19) Spike RBD stained by SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min