- ￥1700 - 4000
- GeneTex
- 美国
- GTX132597
- 2025年07月14日
- WB, ICC/IF
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr467 of human PI3 kinase p85. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX132597
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF
- 浓度：
1.02 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199)
- 抗体英文名：
PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody
- 抗体名：
PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody (GTX132597) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Chen M et al., iScience 2023 (PMID:37123238)
Li M et al., Dis Markers 2022 (PMID:35978887)
Jing Chen et al., Front Oncol 2022 (PMID:35372075)
Guichuan Huang et al., Mol Med Rep 2021 (PMID:34080651)
Meng Li et al., Dis Markers 2021 (PMID:34306252)
Yang L et al., Front Pharmacol 2021 (PMID:34955834)
Chiou TT et al., Biomed Pharmacother 2021 (PMID:34700229)
Luo X et al., Cell Death Dis 2021 (PMID:33771982)
Li J et al., J Ethnopharmacol 2020 (PMID:32344236)
Chao CC et al., Cancers (Basel) 2020 (PMID:32079335)
Li H et al., Neurotox Res 2020 (PMID:31970651)
Jau?Tien Lin et al., Journal of Food Biochemistry 2019 (Epub)
Fang L et al., Int J Mol Sci 2019 (PMID:30781615)
Huifang Yan et al., Evidence-Based Complementary and Alternative Medicine 2018 2018()
Lin JT et al., Journal of Functional Foods 2018 47()
Ali NA et al., Breast Cancer Res 2014 (PMID:25200860)
Chiu WC et al., PLoS One 2016 (PMID:27875549)
signaling公司的Phospho-PI3K p85 (Tyr458)/p55(Tyr199) 我自己分析没有条带无非是细胞状态不好，或者抗体不好，不知道还有没有可能是没有加磷酸化酶抑制剂，以至于磷酸化的蛋白被去磷酸化了？ 谢谢啦～ yhwangcams 检测内参蛋白如GAPDH,ACTIN,TUBULIN是否成功？ 用PLB裂解细胞得到的蛋白适合做REPORTER，做WESTERN不太合适。建议可以用其他BUFFER裂解蛋白。
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 antibody (A) and Total-p38 antibody (B). Cell Based Elisa were performed directly in the 96-well plates using Phospho-p38 and Total-p38 antibodies (C). Figure 3: JNK detection with FACECells were serum-starved for 16 hours
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