- ￥5300
- GeneTex
- 美国
- GTX17578
- 2025年07月15日
- ELISA, Sandwich ELISA
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Glycosylated, CHO-derived recombinant human GM-CSF
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Biotin
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX17578
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
ELISA, Sandwich ELISA
- 浓度：
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
GM-CSF
- 抗体英文名：
GM-CSF antibody [1089] (Biotin)
- 抗体名：
GM-CSF 抗体 [1089] (Biotin)
- 规格：
250 μg
ELISA analysis of human GM-CSF recombinant protein using GTX17578 GM-CSF antibody [1089] (Biotin).
Protein concentration : 0, 15.4, 38.4, 96, 240, and 600 pg/ml (both in quadruplicate)
Capture : GTX17576(9 μg/mL)
Detection : GTX17578 (0.05 μg/mL)
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文献和实验The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room
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