- ￥4700
- GeneTex
- 美国
- GTX02066
- 2025年07月09日
- WB, FACS
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant Human MMP-2 protein
- 亚型：
IgG2a
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX02066
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, FACS
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
No cross-reactivity with human MMP1 protein, human MMP7 protein, and human MMP9 protein in ELISA assay.
- 抗体英文名：
MMP2 antibody [05]
- 抗体名：
MMP2 抗体 [05]
- 规格：
100 μl
WB analysis of A549 whole cell lysate (Lane A) using GTX02066 MMP2 antibody [05].
Dilution : 1:500
Loading : 30 μg
FACS analysis of MG-63 cells using GTX02066 MMP2 antibody [05].
The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
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文献和实验to 0.05, and zymolyase (100T) to 60 ug/ml. Incubate 1 hour at 37oC. Pellet spheroplasts as above. Resuspend in 10 mls Solution A and pellet again. Combine the suspension of whole cells with the spheroplasts and pellet as above. Resuspend in 10
Conjugation of Antibody to HRP
Reagents and Materials PBS PBS: 0.05M EDTA PBS: 0.01 thimerosal Conjugate Storage Buffer SATA DMF Hydroxylamine hydrochloride NaOH Pellets PD10 Column or Sephadex G25 Sulfo-SMCC Preparation of Thiolated
Antibody cleanup In order to decrease the amount of nonspecific staining, it is often necessary to preabsorb primary and secondary antibodies to yeast cells lacking the antigen prior to use. A 1:1 mixture of fixed yeast
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