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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant Rab6AQ72L, a GTP-locked mutant of Rab6A in which Gln72 is replaced by Leu.
- 亚型:
IgG2
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Drosophila
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX33942
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Human
- 应用范围:
ICC/IF
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Recognizes human, mouse and Drosophila GTP-bound Rab6a and Rab6b and mutant Rab6Q72L. Does not detect Rab6-GDP.
- 抗体英文名:
RAB6 (Q72L mutant specific) antibody [AA2]
- 抗体名:
RAB6 (Q72L mutant specific) 抗体 [AA2]
- 规格:
100 μg
Rab6-GTP is detected by immunocytochemistry using anti-Rab6-GTP, mAb (AA2). Method: HeLa cells are grown in standard culture conditions, fixed with PFA (3%), permeablized in PBS+ BSA 0.2 % + Saponin 0.05 % and incubated with anti-Rab6-GTP, mAb (AA2) (1μg /ml) in PBS-BSA-Saponin. After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody in PBS-BSA-Saponin for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.
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文献和实验Identification of a Mutant Kinase/ATP Analog Pair
of the mutant kinase to phosphorylate a substrate with analog ATP. Doing this requires either radiolabeled ATP analogs or, preferably, a phospho-specific antibody to the phosphorylation site on the known substrate. In our experiments, we used a commercially
Antibody Affinity Maturation by Chain Shuffling
Phage display can be used to increase the affinity of antibodies more than 1,000-fold (1 ,2 ). The starting point is typically a specific antibody isolated from a phage antibody library (see Chapter 8 ). To accomplish affinity maturation
DamIP: Using Mutant DNA Adenine Methyltransferase to Study DNA‐Protein Interactions In Vivo
. This method is simple and does not require protein?DNA crosslinking or a specific antibody to the protein of interest. This unit describes the application of this method for identification of DNA binding sites in vivo. Curr. Protoc. Mol. Biol. 94:21.21.1?21.21
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