- ￥5300
- GeneTex
- 美国
- GTX57553
- 2025年07月15日
- WB, IHC-P, FACS
- Mouse
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The clone 5A10 is derived from hybridization of mouse F2 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Nanog protein.
- 亚型：
IgG2a
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX57553
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, IHC-P, FACS
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Nanog
- 抗体英文名：
Nanog antibody [5A10]
- 抗体名：
Nanog 抗体 [5A10]
- 规格：
100 μl
IHC-P analysis of human seminoma tissue using GTX57553 Nanog antibody.
Antigen retrieval: 0.1M sodium citrate buffer
Dilution: 1:50
FACS analysis of Hep3B cells using GTX57553 Nanog antibody.
Cell Number: 1 x 10⁶ cells
Upper panel : negative control, Lower panel: Primary antibody
Antibody amount: 2-5 μg
WB analysis of NIH3T3 whole cell lysate using GTX57553 Nanog antibody.
Loading : 35 μg
Dilution : 1:500
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文献和实验ELISPOT (Enzyme-linked ImmunoSPOT)
of cytokine-producing cells at the single cell level. The method employs the sandwich assay approach of the enzyme-linked immunosorbent assay (ELISA), with some variations. The ELISPOT capture antibody is coated aseptically onto a polyvinylidene difluoride
Neutralizing Bioassay Protocols
describe in vitro bioassays using neutralizing anti-mouse and anti-human cytokine antibodies. In general, the cytokine bioassay protocols are modified to pre-incubate the cytokine of interest with the specific neutralizing antibody prior to addition
Intracellular Immunofluorescent Staining for Flow Cytometry
Guide Mouse Cytokines: Intracellular Staining Quick Guide Mouse Cytokine Cell Source Activation Incubation Time Restimulation Intracellular Block Antibody
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