- ￥5300
- GeneTex
- 美国
- GTX57559
- 2025年07月07日
- WB, ICC/IF, FACS
- Mouse
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The clone 4E11 is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human SNAP27 protein.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX57559
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, FACS
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
SNAP25
- 抗体英文名：
SNAP25 antibody [4E11]
- 抗体名：
SNAP25 抗体 [4E11]
- 规格：
100 μl
WB analysis of Mouse brain tissue lysate using GTX57559 SNAP25 antibody.
Loading : 30 μg
Dilution : 1:2000
ICC/IF analysis of U87-MG cells using GTX57559 SNAP25 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
FACS analysis of U87-MG cells using GTX57559 SNAP25 antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg
WB analysis of various samples using GTX57559 SNAP25 antibody.
Lane 1 : 293T cell lysate
Lane 2 : SNAP25 transfected 293T cell lysate
Loading : 10 μg
Dilution : 1:3000
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文献和实验Hematopoietic Stem Cell Enrichment From the AGM Region of the Mouse Embryo
in the intraembryonic aorta-gonad-mesonephros region at embryonic day (E) 10.5. After E11, these HSCs are thought to seed the liver and then the bone marrow just before birth. So far, many questions concerning the origin, properties, and functionality of these HSCs
Wholemount in situ hybridisation
. Each batch should ideally be tested. As a rough guide, use 5 min for E7.5, 7 min for E8.5, 9 min for E9.5, 11 min for E10.5, 12-14 min for E11.5. 8 Wash twice with PBTX for 5 minutes each. Be careful - the embryos are fragile
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