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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys4)
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX60878
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, ELISA, ChIP assay
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
This antibody reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in histone H3
- 抗体英文名:
Histone H3K4me1 (Mono-methyl Lys4) antibody [RM140]
- 抗体名:
Histone H3K4me1 (Mono-methyl Lys4) 抗体 [RM140]
- 规格:
100 μg
ChIP analysis of HeLa cells using GTX60878 Histone H3K4me1 (Mono-methyl Lys4) antibody [RM140]. Real-time PCR was performed using primers specific to the gene indicated.
Antibody amount : 5μg
WB analysis of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2) using GTX60878 Histone H3K4me1 (Mono-methyl Lys4) antibody [RM140].
Dilution : 0.5μg/ml
ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60878 Histone H3K4me1 (Mono-methyl Lys4) antibody [RM140].
Red : Primary antibody
Green : Actin
The GTX60878 reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in histone H3.
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文献和实验," made up of fragments of up to seven nucleosomes in length, is incubated with an antiserum directed against the histone modification of interest. The antibody-bound fraction is separated from the unbound fraction and, after extraction of genomic DNA from the bound
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