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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
The clone AT5C6 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ST15 protein.
- 亚型:
IgG2a
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX57598
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, FACS
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
HIP
- 抗体英文名:
HIP antibody [AT5C6]
- 抗体名:
HIP 抗体 [AT5C6]
- 规格:
100 μl
WB analysis of various samples using GTX57598 HIP antibody.
Lane 1 : 293T whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : SW480 whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : K562 whole cell lysate
Lane 6 : LNCap whole cell lysate
Lane 7 : HeLa whole cell lysate
Lane 8 : PC-3 whole cell lysate
Loading : 40 μg
Dilution : 1:1000
ICC/IF analysis of 293T cells using GTX57598 HIP antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
ICC/IF analysis of A549 cells using GTX57598 HIP antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
FACS analysis of Hep3B cells using GTX57598 HIP antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg
FACS analysis of 293T cells using GTX57598 HIP antibody.
Cell Number: 1 x 10⁶ cells
Red: Primary antibody
Antibody amount: 2-5 μg
ICC/IF analysis of HeLa cells using GTX57598 HIP antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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文献和实验在做对小鼠成纤维细胞的整个基因组的甲基化水平检测,我是想要关于免疫化学法中提及的关于anti-m5C的一抗和荧光标记的二抗的选择。我不知道怎么选择抗体。希望有这方面经验的专家或者有文献的前辈给我发一下吧。非常感谢。很急!!!!还要谢谢前面那位前辈。非常感谢。 藤崽 你说的是全基因组甲基化抗体吧,就是抗5-methyl cytosine的抗体,很多公司都有的,你查一下,我们用的是abcam公司的,四千左右,二抗就是普通的国产就行了
Immunofluorescent staining of Sea Urchin embryos
that there are embryos. Coverslip. Examine using epifluorescence. Antibodies from David McClay Ig8, 1D5 -PMCs and spicules Ecto V, 295 - ventral ectoderm 5C7 - vegetal plate and posterior archenteron Secondary antibody Texas Red
Cloning and Expression of Single-Chain Fragments (scFv) from Mouse and Rat Hybridomas
For cloning and expressing the antigen-binding variable (Fv) portion of an antibody in Escherzchia coli , vectors have been constructed that combine the two variable regions (VH and VL ) with a peptide linker (1 –3 ). The genetic
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