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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Mouse sperm
- 亚型:
IgG2a
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Mouse
- 目录编号:
GTX00690
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rat
- 应用范围:
WB, ICC/IF
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
This antibody showed no reactivity with ADAM1A (confirmed by WB using recombinant ADAM1A and ADAM1B proteins).
- 抗体英文名:
ADAM1B antibody [#158]
- 抗体名:
ADAM1B 抗体 [#158]
- 规格:
100 μg
WB analysis of mouse testis lysates collected from different ages using GTX00690 ADAM1B antibody [#158].
ADAM1B appeared as a faint signal at 3 weeks of age.
ICC/IF analysis of fresh mouse sperms using GTX00690 ADAM1B antibody [#158].
Dilution : 1:300
Fixation : 4% PFA
WB analysis of various mouse tissue samples using GTX00690 ADAM1B antibody [#158]. The Adam1B synthesized in testis (120 kDa) is proteolytically processed into smaller form (60 kDa) in sperm during the epididymal maturation of the spermatozoa. By western blotting, ADAM1B is detected in testis extract at 120 kDa position, which is due to glycosylation and larger than predicted size of 89 kDa from the sequence.
This protien is testis-specific.
Dilution : 1:100
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文献和实验Ikawa M et al., J Biol Chem. 2011 (PMID:21131354)
Yamaguchi R et al., Mol Biol Cell. 2012 (PMID:22621904)
Tokuhiro K et al., Proc Natl Acad Sci U S A. 2012 (PMID:22357757)
Antibody-Induced Opsonophagocytosis of Serogroup B Meningococci Measured by Flow Cytometry
meningococci, there seems to be a correlation between SBA activity and protection (1 ), whereas OP has been less well-studied. This function may have a supplementary or even major role in protection against group B meningococci.
Buffer and save the flowthrough.(12) Wash with 6 ml Buffer A, 6 ml Buffer B, and 6 ml Buffer A. Hold at room temperature for 30 minutes.(13) Wash with 6 ml Buffer B, 6 ml Buffer A, and 6 ml Buffer B. Wash with Storage Buffer and hold at 4°C.(14) To purify
this method will dilute the antibody by a factor of 2. Using a PD-10 column: a)Wash PD-10 column with 30mL of PBS. b) Add the 1mL FITC-Ig or Biotin Ig solution after the top of column is almost dry. Close stopcock to stop flow after the Antibody
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