- ￥1700 - 4000
- GeneTex
- 美国
- GTX633409
- 2025年07月10日
- WB, ICC/IF, IHC-P
- Mouse
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide surrounding acetyl Lys20 of human Histone H2B. The exact sequence is proprietary.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX633409
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P
- 浓度：
1.1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Histone H2BK20ac (acetyl Lys20)
- 抗体英文名：
Histone H2BK20ac (acetyl Lys20) antibody [GT578]
- 抗体名：
Histone H2BK20ac (acetyl Lys20) 抗体 [GT578]
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Histone H2BK20ac (acetyl Lys20) antibody [GT578] detects Histone H2BK20ac (acetyl Lys20) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Histone H2BK20ac (acetyl Lys20) stained by Histone H2BK20ac (acetyl Lys20) antibody [GT578] (GTX633409) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2BK20ac (acetyl Lys20) antibody [GT578] (GTX633409) diluted at 1:1000.
Histone H2BK20ac (acetyl Lys20) antibody [GT578] detects Histone H2BK20ac (acetyl Lys20) protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: Histone H2BK20ac (acetyl Lys20) stained by Histone H2BK20ac (acetyl Lys20) antibody [GT578] (GTX633409) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 μm.
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dishes of lysed E. coli to reduce anti-E. coli antibody background. These antibodies are present in all human sera, rabbit sera, and rodent sera. For each Petri dish containing solidified LB agar, mix 0.6ml of the Y1090 overnight-saturated culture with 30
-Histone H3 和 MBD1 条带相同，简单调了下对比度；Ac-Histone H4 和 HDAC1 条带相同，180 度旋转即可。（同一个图中出现相同条带，能不能长点儿心，别把读者当傻子。。。）图片来源于论文第二篇文章于 2011 年发表，2018 年遭撤稿，目前已经被引用 118 次。图片来源于 pubmed这篇文章撤稿的主要原因同样是 WB 条带重复利用，不过这次作者聪明多了，在不同的图中出现相同条带，甚至将之前发表过的图重新利用。眼尖的小伙伴看出来了吗，3E 和 5B 的β-actin 相同
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