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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthetic peptide corresponding to amino acid residues near the N-terminus of Hb β-subunit conjugated to KLH.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX04269
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ELISA, Lateral Flow
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
This antibody detect the E6K mutant in the hemoglobin beta subunit and does not react with the HbA, HbS, HbF, or HbA-2 isoforms.
- 抗体英文名:
Hemoglobin beta C antibody [15C2.C11.F2.G11]
- 抗体名:
Hemoglobin beta C 抗体 [15C2.C11.F2.G11]
- 规格:
100 μg
WB analysis of various sample lysates using GTX04269 Hemoglobin beta C antibody [15C2.C11.F2.G11].
Lane 1 : Prestained molecular weight marker
Lane 2 : HbA peptide conjugated to BSA
Lane 3 : HbA-2 peptide conjugated to BSA
Lane 4 : HbC peptide conjugated to BSA
Lane 5 : HbF peptide conjugated to BSA
Lane 6 : HbS peptide conjugated to BSA
Lane 7 : BSA alone
Loading : 50ng per lane
Dilution : 1μg/mL
ELISA analysis of 0.1μg BSA-conjugated HbC peptide using GTX04269 Hemoglobin beta C antibody [15C2.C11.F2.G11]. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody.
Purple : GTX04269
Green : Hemoglobin polyclonal antibody
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文献和实验of beta-mercaptoethanol to 0.1%, glusulase to 0.05%, and zymolyase (100T) to 60 ug/ml. Incubate 1 hour at 37o C. Pellet spheroplasts as above. Resuspend in 10 mls Solution A and pellet again. Combine the suspension of whole cells
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Integrating extrachromasomal arrays into the C. elegans chromosomes Why and how to do it by Michael Koelle What is the benefit of integrating an extrachromosomal array
, or as often as necessary to decrease the background. Pellet cells. Save antibody supernatant, which is now diluted 1:5 from its initial concentration. These antibodies can be stored at 4o C for several weeks or at -70o C for longer periods. Solutions
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